The work behind the platform.

Papers

• Analytical Chemistry2026

  Chemical8 dosesPhosphoN-glycoProteome

  Dose-Dependent Inhibition of Protein Glycosylation Reveals Crosstalk between Glycosylation and Phosphorylation in EGF Signaling Pathways

  Liu H, Lin DC, Hu Y, Sun Z, Huang Y, Xu Y, Zhang H.

  Tunicamycin at eight doses, with and without EGF, in A498 kidney cancer cells. 215 EGF-regulated phosphopeptides changed with dose across ERBB, MAPK and mTOR signalling; glycopeptides including EGFR Asn568 tracked downstream phosphorylation.

  98(12):9293–9304doi:10.1021/acs.analchem.5c08113PXD071440PMC13044880
• Analytical Chemistry2026

  GeneticPhosphoN-glycoProteome

  Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling

  Wei E, Liu H, Betenbaugh M, Zhang H.

  Multiplex CRISPR glycoengineering of HEK293 cells to a defined glycome, across three serum states: over 10,900 proteins and up to 38,772 phosphosites per data set. Engineered cells rewired serum signalling toward calcium/PLC-linked and cell-cycle programmes.

  98(25):18577–18595doi:10.1021/acs.analchem.6c00790PXD071742PMC13352514
• Research Square2026 · preprint, under review

  EnzymaticPhosphoN-glycoProteome

  Attenuation of EGFR-Mediated Cellular Signaling by Sialidase-Mediated Desialylation

  Liu H, Wei E, Lin DC, Zhang H.

  Enzymatic desialylation, with and without an EGFR inhibitor, in A498 cells. Losing sialylated glycoforms at EGFR N413 and N444 went with lower phosphorylation at Y1197; over 1,500 phosphosites were regulated only under the combined treatment.

  posted 2026-07-02doi:10.21203/rs.3.rs-10144726/v1PXD078365 (released on publication)

Author lists as published. Raw data for the two published studies is public in PRIDE; PXD078365 is released on publication.